Repeated Cycles of amplification bring on degradation from the probe, resulting in enhanced fluorescence. TaqMan detection is, thus, much more distinct than dye‐centered detection, simply because an increase in fluorescence occurs only upon binding in the probe to a particular sequence and subsequent de‐quenching, even though no signal is produced https://www.facebook.com/NewTechFind/posts/pfbid02oAUGPQdYY2M4MohD8hMLNPGCE3jXSg9wdixptBpxnhX8sjg8J18oSvADn5EpCmLtl